UV-Visible Spectroscopy (UV-Vis) is a widely used analytical technique that utilizes the absorption of light in the ultraviolet and visible regions of the electromagnetic spectrum to obtain information about the electronic structure and chemical composition of a sample.
The basic components of a UV-Vis spectrophotometer include a light source that emits radiation in the UV and visible regions of the spectrum, a monochromator that selects a specific wavelength of light, a sample holder that contains the sample to be analyzed, and a detector that measures the intensity of the light that passes through the sample.
UV-Vis spectroscopy is commonly used in a variety of fields, including chemistry, biochemistry, materials science, and environmental science, to determine the concentration of a substance in a solution, to study the kinetics of a reaction, or to identify the presence of certain functional groups in a molecule. UV-Vis spectroscopy is a powerful analytical tool that is easy to use, relatively inexpensive, and widely available in many laboratories.
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